Ecocyte mRNA & cDNA Injection service

Injected oocytes can also be used for a number of experimental assays in which expression of membrane proteins must be analysed, including studies of ion channel mutants, pharmacological studies and transporter kinetics. We therefore provide oocytes injected with cDNA or cRNA. You can send us your mRNAs for wild-type or mutant variants, or we procure the DNA and do the in vitro transcription for you. We then inject the number of oocytes requested and send them back with our express delivery service, ready to be analyzed after heterologous expression. We inject all oocytes by use of the Roboocyte® TEVC and Injection automate (Multichannelsystems, Reutlingen, Germany). You can also tag the RNA with GFP in case that you want to control the expression under the fluorescent microscope, or other tags for further downstream applications.

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